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Online First

10.4103/aja202425

Assessing the efficacy of a novel sperm-washing medium enriched with serotonin, L-carnitine, and coenzyme Q10: an observational cohort study

Dogan, Sinem1; Aydin, Turgut2; Koroglu, Nadiye2,3; Yilmazer, Yasemin4; Albayrak, Nazli2; Cetin, Fadime5; Moshfeghi, Elnaz6; Celik, Ozge1

1Department of Molecular Biology and Genetics, Istanbul Kultur University, Istanbul 34158, Türkiye

2Department of Obstetrics and Gynecology, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul 34303, Türkiye

3Department of Obstetrics and Gynecology, School of Medicine, Istanbul Beykent University, Istanbul 34500, Türkiye

4Department of Molecular Biology and Genetics, Istanbul Sabahattin Zaim University, Istanbul 34303, Türkiye

5Department of Bioengineering, Istanbul Yildiz Technical University, Istanbul 34349, Türkiye

6Department of Molecular Biology and Genetics, Istanbul Yildiz Technical University, Istanbul 34349, Türkiye

Correspondence: Dr. S Dogan (sinemercandogan@gmail.com)

Originally published: June 11, 2024 Received: November 25, 2023 Accepted: March 14, 2024

Abstract

This observational cohort study investigated the potential of a novel sperm-washing medium (SWM) enriched with serotonin (5-HT), L-carnitine (L-C), and coenzyme Q10 (CoQ10) to enhance sperm motility and reduce DNA damage. It compared this innovative medium (5-HT/L-C/CoQ10 SWM) with two widely used commercial media (SWM 1 and SWM 2). Ninety-eight volunteers from an infertility clinic provided semen samples, which were divided into three aliquots for analysis in different SWMs: group 1, SWM was composed of hydroxyethyl piperazineethanesulfonic acid (HEPES), sodium bicarbonate, human serum albumin (HSA), taurine, and gentamicin sulfate (SWM 1); group 2, SWM was composed of HEPES, sodium bicarbonate, and HSA (SWM 2); and group 3, SWM was composed of HEPES-buffered human tubal fluid supplemented with 5-HT, L-C, and CoQ10 (5-HT/L-C/CoQ10 SWM). Sperm motility was categorized as progressive, nonprogressive, or immotile. Apoptosis, reactive oxygen species (ROS) production, and DNA fragmentation were also assessed. There were no significant differences in total or progressive sperm motility among the groups. Spermatozoa in group 3 exhibited reduced apoptosis, necrosis, and ROS levels and increased viability. No significant differences were observed in the DNA fragmentation index among groups. The 5-HT/L-C/CoQ10 SWM reduced sperm oxidative stress and apoptosis compared with those of the two commercially available SWMs, suggesting that 5-HT/L-C/CoQ10 SWM could be useful for enhancing in vitro fertilization success rates.

Keywords: apoptosis; coenzyme Q10; DNA fragmentation; L-carnitine; serotonin; sperm-washing medium

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.