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中文摘要

《亚洲男性学杂志》 2010; 12 (5): 753-759

精液制备技术和培养条件对精子DNA完整性的影响

恰当的精液处理和评估是成功治疗不孕症的关键。本文研究精液化验程序(包括制备和培养)是否会影响精子DNA的完整性。研究对象包括153名不育男性。在不同条件下处理和培养新鲜射出的精液样本,检测常规的精液参数、精子染色质结构分析(SCSA)参数(DNA断裂指数,%DFI;DNA着色比例,%HDS)。%DFI与精子浓度、活动力、前向运动力之间呈负相关。与单独梯度离心(DGC)相比,随后进行了上游处理的精子的%DFI较低(P < 0.01)。尽管精子在室温下和37ºC培养的精子的%DFI都随培养时间的延长而升高,但室温下培养24小时后精子%DFI明显比37ºC下低(P < 0.05)。加5% CO2培养后的精子活力明显得到保持(P < 0.01),然而它又进一步增加了%DFI(P < 0.001)。因此,精子DNA损伤与延长培养时间有关。有趣的是,常规的培养条件,比如保持原有PH和温度,也对精子DNA完整性有损。

关键词:梯度离心,DNA损伤,男性不育,精子染色质结构分析,精子

 
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