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中文摘要

《亚洲男性学杂志》 2008; 10 (4): 577-584

《亚洲男科学杂志》 2008; 10(4): 577-584

【论著】使用抗nectin-2和抗nectin-3抗体来研究Sertoli细胞和成熟精子细胞之间外胚层特化的分布

Yoshiro Toyama, Fumie Suzuki-Toyota, Mamiko Maekawa, Chizuru Ito, Kiyotaka Toshimori(日本)

目的:了解在生精上皮中Sertoli细胞和成熟精子细胞之间外胚层特化的生物学功能。方法:为了阻断外胚层特化的功能,特化中表达的nectin-2蛋白被显微注射到小鼠生精管腔中的抗nectin-2抗体所中和。抗nectin-3的抗体也被显微注射到管腔中来中和特化中表达的nectin-3蛋白。结果:特化中的肌动蛋白丝消失,光镜下发现成熟的精子细胞脱落。结论:nectin-2和nectin-3蛋白分别被抗nectin-2抗体和抗nectin-3抗体所中和。失活的nectin-2和nectin-3破坏了nectin-afadin-actin系统,最终导致肌动蛋白丝的消失,从而使特化失去了维持功能,出现精子的脱落。特化的功能之一可能是在释放精子前保持成熟精子的状态。

关键词:外胚层特化;Sertoli细胞;精子发生细胞;睾丸;肌动蛋白;nectin;小鼠

 
Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.