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Volume 13, Issue 2 (March 2011) 13, 275–280; 10.1038/aja.2010.147
The role of C-type natriuretic peptide in rat testes during spermatogenesis
Dong-Hui Huang1, Shi-Wei Zhang2, Hu Zhao3 and Ling Zhang1
1 Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
2 Centre Laboratory, Zhongshan Hospital of Hubei Province, Wuhan 430033, China
3 Department of Human Anatomy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Correspondence: Dr H Zhao, (zhaohu@mails.tjmu.edu.cn)
Received 10 December 2009; Revised 16 May 2010; Accepted 20 September 2010; Published online 20 December 2010
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C-type natriuretic peptide (CNP) is a 22-amino acid peptide and act as a local paracrine or autocrine regulator. There is growing evidence that CNP is involved in male reproductive processes. To investigate the role of CNP during spermatogenesis, we measured the mRNA expression of CNP and its specific membrane-bound natriuretic peptide receptor-B (NPR-B) using real-time RT-PCR in the testes of normal rats on different postnatal days. After that spermatogenesis dysfunction model induced by ornidazole was established with the aim to study the correlation of CNP with spermatogenic dysfunction. Then, Sertoli cells from 18- to 22-day-old healthy male rats were cultured in the presence of different CNP concentrations (1×10−6, 1×10−7 and 1×10−8 mol l−1), and the mRNA expression levels of androgen-binding protein, inhibin B and transferrin were examined at 0 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 48 h. During the postnatal development of rat testes, the highest mRNA expression levels of CNP and NPR-B were found at postnatal D0, and the levels then declined gradually, with a second CNP peak at postnatal D35. In the ornidazole-induced infertile rat testes, CNP gene expression was lower than in the uninduced rats (P<0.05), while NPR-B gene expression was greater (P<0.05). In cultured Sertoli cells, supplementation with CNP stimulated the gene expression of androgen-binding protein/inhibin B/transferrin, particularly at 12 h, and 1×10−7 mol l−1 CNP had the highest upregulation effect. The gene expression levels of CNP/NPR-B in rat testes at different postnatal stages and in infertile rat testes indicated that CNP may participate in the physiology and/or pathology related to spermatogenesis. Moreover, CNP regulated endocrine function in Sertoli cells. Taken together, these results showed that CNP is closely tied to spermatogenesis.
Keywords: C-type natriuretic peptide; natriuretic peptide receptor-B; Sertoli cell culture; spermatogenesis
Keywords: C-type natriuretic peptide; natriuretic peptide receptor-B; Sertoli cell culture; spermatogenesis
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