Home  |   Archive  |   Online Submission  |   News & Events  |   Subscribe  |   APFA  |   Society  |   Contact Us  |   中文版

Ahead of print
Authors' Accepted
Current Issue
Special Issues
Browse by Category

Manuscript Submission

Online Submission
Online Review
Instruction for Authors
Instruction for Reviewers
English Corner new!

About AJA

About AJA
Editorial Board
Contact Us

Resources & Services

Email alert

Download area

Copyright licence
EndNote style file
Manuscript word template
Guidance for AJA figures
    preparation (in English)

Guidance for AJA figures
    preparation (in Chinese)

Proof-reading for the

AJA Club (in English)
AJA Club (in Chinese)


Volume 15, Issue 4 (July 2013) 15, 518–522; 10.1038/aja.2013.9

Is thioredoxin reductase involved in the defense against DNA fragmentation in varicocele?

Gül Özdemirler Erata1, Canan Küçükgergin1, Gülsan Aktan2, Ates Kadioglu2, Müjdat Uysal1 and Necla Koçak-Toker1

1 Department of Biochemistry, Istanbul Medical Faculty, University of Istanbul, Çapa, 34093, Istanbul, Turkey
2 Department of Urology, Istanbul Medical Faculty, University of Istanbul, Çapa, 34093, Istanbul, Turkey

Correspondence: Dr G Özdemirler Erata, (gul_erata@yahoo.com)

Received 16 December 2012; Revised 26 December 2012; Accepted 20 January 2013; Advance online publication 22 April 2013


We aimed to investigate the role of thioredoxin reductase (TR) and inducible heat shock protein 70 (iHsp70) and their relationship with sperm quality in varicocele (VAR) patients. Semen samples were obtained from 16 subfertile men diagnosed as VAR and 10 fertile men who applied to the Andrology Laboratory of Istanbul Medical Faculty of Istanbul University. The sperm TR and iHsp 70 expression levels were determined using Western blot analysis. The TR activity of the sperm was assayed spectrophometrically. The sperm quality was evaluated both by conventional sperm analysis and by a terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) technique that assayed DNA-fragmented spermatozoa in semen samples. The percentage of TUNEL-positive spermatozoa in the VAR group (16.3%±5.6%) was higher than that in the fertile group (5.5%±1.9%). Significant inverse correlations were detected between the percentage of TUNEL-positive cells and both the concentration (r=−0.609; P=0.001) and motility (r=−0.550; P=0.004) of spermatozoa. Both the TR expression and activity were increased significantly in the VAR group (U=22.0; P=0.001 and U=33.5; P=0.012, respectively) as analyzed using the Mann–Whitney U Wilcoxon rank sum W test. Furthermore, significant positive correlations were found between TR expression and activity (r=0.406; P=0.040) and between TR expression and the percentage of TUNEL-positive cells (r=0.665; P=0.001). Sperm iHsp70 expression did not differ between the VAR and fertile groups. In conclusion, increased sperm TR expression might be a defense mechanism against apoptosis in the spermatozoa of men with VAR.

Keywords: DNA damage; inducible heat shock protein 70 (iHsp70); thioredoxin reductase (TR); spermatozoa; varicocele

PDF | PDF | 中文摘要 |

Browse:  3357
Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.