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Volume 9, Issue 3 (May 2007) 9, 361–367; 10.1111/j.1745-7262.2007.00265.x
Magnetic resonance evaluation of human mesenchymal stem cells in corpus cavernosa of rats and rabbits
Yun-Seob Song, Ja-Hyeon Ku, Eun-Seop Song, Jung-Hoon Kim, Jin-Suck Jeon, Kong-Hee Lee, Sook-Ja Kim, Hee-Jeong Cheong, Ik-Sung Lim, Dongho Choi and Jong-Ho Won
1.Department of Urology, Stem Cell therapy Center, Soonchunhyang School of Medicine, Seoul 140-743, Korea
2.Department of Urology, Seoul National University Hospital, Seoul 110-744, Korea
3.Department of Obstetrics and Gynecology, Inha University School of Medicine, Incheon 400-103, Korea
4.Department of Radiology, Soonchunhyang School of Medicine, Seoul 140-743, Korea
5.Department of Internal Medicine, Soonchunhyang School of Medicine, Seoul 140-743, Korea
6.Department of Urology, Soonchunhyang School of Medicine, Seoul 140-743, Korea
7.Stem Cell therapy Center, Soonchunhyang School of Medicine, Seoul 140-743, Korea
8.Department of Industrial Management & Engineering, Namseoul University College of Engineering, Cheonan 330-707, Korea
9.Department of Surgery, Stem Cell therapy Center, Soonchunhyang School of Medicine, Seoul 140-743, Korea
10.Department of Internal Medicine, Stem Cell therapy Center, Soonchunhyang School of Medicine, Seoul 140-743, Korea
Correspondence: Dr Jong-Ho Won, Department of Internal Medicine, Stem Cell therapy Center, Soonchunhyang School of Medicine, Seoul 140-743, Korea. Fax: +82-2709-9200. E-mail: jhwon@hosp.sch.ac.kr; Dr Dongho Choi, Department of Surgery, Stem Cell therapy Center, Soonchunh
Received 16 June 2006; Accepted 6 November 2006.
| Abstract |
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Aim: To investigate whether the biological process of superparamagnetic iron oxide (SPIO)-labeled human mesenchymal stem cells (hMSCs) may be monitored non-invasively by using in vivo magnetic resonance (MR) imaging with conventional 1.5-T system examinations in corpus cavernosa of rats and rabbits.
Methods: The labeling efficiency and viability of SPIO-labeled hMSCs were examined with Prussian blue and Tripan blue, respectively. After SPIO-labeled hMSCs were transplanted to the corpus cavernosa of rats and rabbits, serial T2-weighted MR images were taken and histological examinations were carried out over a 4-week period.
Results: hMSCs loaded with SPIO compared to unlabeled cells had a similar viability. For SPIO-labeled hMSCs more than 1 105 concentration in vitro, MR images showed a decrease in signal intensity. MR signal intensity at the areas of SPIO-labeled hMSCs in the rat and rabbit corpus cavernosa decreased and was confined locally. After injection of SPIO-labeled hMSCs into the corpus cavernosum, MR imaging demonstrated that hMSCs could be seen for at least 12 weeks after injection. The presence of iron was confirmed with Prussian blue staining in histological sections.
Conclusion: SPIO-labeled hMSCs in corpus cavernosa of rats and rabbits can be evaluated non-invasively by molecular MR imaging. Our findings suggest that MR imaging has the ability to test the long-term therapeutic potential of hMSCs in animals in the setting of erectile dysfunction.
Keywords: human mesenchymal stem cells, magnetic resonance, stem cells, penis, cell labeling, corpus cavernosa
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