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Volume 9, Issue 1 (January 2007) 9, 77–81; 10.1111/j.1745-7262.2007.00219.x
Influence of enterococci on human sperm membrane in vitro
Hua Qiang, Ming-Sen Jiang, Jian-Yin Lin and Wei-Min He
1.Department of Pathogenic Biology Etiology, School of Medicine, Wuhan University, Wuhan 430071, China
2.Department of Etiology, Histology and Embryology, Fujian Medical University, Fuzhou 350004, China
3.Department of Anatomy, Histology and Embryology, Fujian Medical University, Fuzhou 350004, China
Correspondence: Dr Hua Qiang, Department of Etiology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, Fujian 350004, China. E-mail: qianghua66@sina.com
Received 17 May 2006; Accepted 5 June 2006.
| Abstract |
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Aim: To study the influence of enterococci on human sperm membrane in vitro.
Methods: Ejaculated human sperm were artificially infected with -hemolytic or non--hemolytic enterococci at the bacteria: sperm ratio of 50:1 at 37°C. Sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling (HOS) test and electron microscopy.
Results: Sperm infected with -hemolytic enterococci had lower HOS scores compared with non--hemolytic strains or uninfected control (P < 0.01). The HOS test scores of sperm infected with -hemolytic enterococci increased in the presence of phosphatidylcholine, an inhibitor of hemolysin. Non--hemolytic strains showed no significant difference in swelling rate, compared with the control group (P > 0.05). It was shown by electron microscopy that -hemolytic enterococci caused significant rupture of human sperm membrane.
Conclusion: -hemolytic enterococci caused human sperm membrane injury, and might be mediated by the hemolysin of enterococci.
Keywords: enterococci, sperm, sperm membrane, hypoosmotic swelling test, ultrastructure
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