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Volume 9, Issue 5 (September 2007) 9, 720–722; 10.1111/j.1745-7262.2007.00274.x
Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood
Rima Dada, Rakesh Kumar, M B Shamsi, Rajeev Kumar, Kiran Kucheria, Raj K Sharma, Satish K Gupta and Narmada P Gupta
1.Laboratory for Molecular Reproduction and Genetics, Anatomy Department, All India Institute of Medical Sciences, New Delhi 29, India
2.Urology Department, All India Institute of Medical Sciences, New Delhi 29, India
3.Endocrinology Department, All India Institute of Medical Sciences, New Delhi 29, India
4.ART Centre, Army Research and Referral Hospital, New Delhi 57, India
5.Gamete Antigen Laboratory, National Institute of Immunology, New Delhi 67, India
Correspondence: Rima Dada, MD, PhD (Genetics), MAMS, Laboratory For Molecular Reproduction and Genetics, Anatomy Department, All India Institute of Medical Sciences (AIIMS), New Delhi 29, India. Fax: +91-11-2658-8663. E-mail: rima_dada@rediffmail.com
Received 29 September 2006; Accepted 8 February 2007.
| Abstract |
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Aim: To determine if Yq microdeletion frequency and loci of deletion are similar in two tissues (blood and sperm) of different embryological origin.
Methods: The present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction (PCR) microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker.
Results: Only 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA.
Conclusion: The frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques (ART), Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.
Keywords: assisted reproductive techniques, infertility, semen, genomic DNA, Yq screening
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