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Abstract

Volume 17, Issue 5 (September 2015) 17, 831–838; doi: 10.4103/1008-682X.146972

Re-epithelialization resulted from prostate basal cells in canine prostatic urethra may represent the ideal healing method after two-micron laser resection of the prostate

Ying Cao1,2,3, Guang-Heng Luo2, Lei Luo2, Xiu-Shu Yang2, Jian-Xin Hu2, Hua Shi2, Ping Huang3, Zhao-Lin Sun2, Shu-Jie Xia1

1 Department of Urology, Shanghai First People’s Hospital, Shanghai Jiaotong University, Shanghai 200080, China;
2 Department of Urology; Guizhou Provincial People’s Hospital, Guiyang 550002, China;
3 Department of Pathology, Guizhou Provincial People’s Hospital, Guiyang 550002, China

Correspondence: Dr. SJ Xia (xsjurologist@163.com) or Dr. ZL Sun (szl@5055.cn)

2015-1-13

Abstract

The purpose of this study is to characterize the re‑epithelialization of wound healing in canine prostatic urethra and to evaluate the effect of this re‑epithelialization way after two‑micron laser resection of the prostate (TmLRP). TmLRP and partial bladder neck mucosa were performed in 15 healthy adult male crossbred canines. Wound specimens were harvested at 3 days, and 1, 2, 3, and 4 weeks after operation, respectively. The histopathologic characteristics were observed by hematoxylin and eosin staining. The expression of cytokeratin 14 (CK14), CK5, CK18, synaptophysin (Syn), chromogranin A (CgA), uroplakin, transforming growth factor‑β1 (TGF‑β1), and TGF‑β type II receptor in prostatic urethra wound were examined by immunohistochemistry and real‑time polymerase chain reaction, respectively. Van Gieson staining was performed to determine the expression of collagen fibers in prostatic urethra and bladder neck would. The results showed that the re‑epithelialization of the prostatic urethra resulted from the mobilization of proliferating epithelial cells from residual prostate tissue under the wound. The proliferating cells expressed CK14, CK5, but not CK18, Syn, and CgA and re‑epithelialize expressed uroplakin since 3 weeks. There were enhanced TGF‑β1 and TGF‑β type II receptor expression in proliferating cells and regenerated cells, which correlated with specific phases of re‑epithelialization. Compared with the re‑epithelialization of the bladder neck, re‑epithelialization of canine prostatic urethra was faster, and the expression of collagen fibers was relatively low. In conclusion, re‑epithelialization in canine prostatic urethra resulted from prostate basal cells after TmLRP and this re‑epithelialization way may represent the ideal healing method from anatomic repair to functional recovery after injury.

Keywords: basal cell; benign prostate hyperplasia; re‑epithelialization; two‑micron laser; wound healing

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Asian Journal of Andrology CN 31-1795/R ISSN 1008-682X  Copyright © 2023  Shanghai Materia Medica, Chinese Academy of Sciences.  All rights reserved.