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Volume 2, Issue 2 (June 2000) 2, 99–102;

Effects of flavonoids from Semen Cuscutae on the reproductive system in male rats

D.N. Qin, B.R. She, Y.C. She, J.H. Wang

Department of Physiology, Shantou University School of Medicine, Shantou 515031, China

Advance online publication 1 June 2000


Aim: To evaluate the effects of the flavonoids extracted from the Semen Cuscutae (FSC) on the reproductive and endocrine functions in male rats. Methods: (1) FSC were obtained from the semen of Cuscuta sinensis Lam through solvent extraction and polyamide columnar chromatography; (2) Effect of FSC on the reproductive organs was assessed in immature rats. Rats were administered FSC through gastric gavage at a dose of 300 mg/kg per day for 7 days and the weights of testis, epididymis, seminal vesicle and pituitary gland were then observed; (3) To observe the effect of FSC on the reproductive endocrine function: same dose level of FSC was given to male rats of different age groups for 7 days; on day 8, the plasma testosterone (T), estradiol (E2) and LH were determined by RIA, the specific binding of LH was estimated and the testes were weighed. (4) Effect of FSC on LH secretion was assessed in vitro on cultured adenohypophysis. (5) Effect of FSC on T secretion was assessed in vitro on Leydig cell culture. Results: FSC increased the weights of testis, epididymis and pituitary gland, and stimulated T and LH secretion both in vitro and in immature rats. Conclusion: FSC invigorates the reproductive system and reproductive endocrine function in male rats.

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