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Volume 4, Issue 2 (June 2002) 4, 111–115;
Microdeletions in the Y chromosome of patients with idiopathic azoospermia
A. Shimizu, T. Ichikawa, N. Suzuki, T. Yamazaki, T. Imamoto, S. Kojima, Y. Naya, A. Komiya, H. Suzuki, K. Nagao, K. Miura, H. Ito
1.Department of Urology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
2.First Department of Urology, Toho University School of Medicine, Tokyo 143-8540, Japan
Advance online publication 1 June 2002
| Abstract |
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Aim: To evaluate the occurrence and prevalence of microdeletions in the Y chromosome of patients with azoospermia. Methods: DNA from 29 men with idiopathic azoospermia was screened by polymerase chain reaction (PCR) analysis with a set of Y chromosome specific sequence-tagged sites (STSs) to determine microdeletions in the Y chromosome. Results: Deletions in the DAZ (deleted in azoospermia) loci sY254 and sY255 were found in three patients with idiopathic azoospermia, resulting in an estimated frequency of deletions of 10.7% in idiopathic azoospermia men. Conclusion : We conclude that PCR analysis is useful for the diagnosis of microdeletions in the Y chromosome, which is important when deciding the suitability of a patient for assisted reproductive technology such as testicular sperm extracion-intracytoplasmic sperm injection (TESE-ICSI).
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