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Volume 4, Issue 2 (June 2002) 4, 131–136;
Suppression of metastasis of rat prostate cancer by introduction of human chromosome 13
S. Hosoki, S. Ota, Y. Ichikawa, H. Suzuki, T. Ueda, Y. Naya, K. Akakura, T. Igarashi, M. Oshimura, N. Nihei, J.C. Barrett, T. Ichikawa, H. Ito
1.Department of Urology, 3Department of Molecular Oncology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan
2.Department of Urology, Teikyo University School of Medicine, Ichihara Hospital, Ichihara 299-0111, Japan
4.Department of Molecular and Cell Genetics, Tottori University School of Medicine, Yonago 683-8503, Japan
5.Laboratory of Biosystems and Cancer, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
Advance online publication 1 June 2002
| Abstract |
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Aim: Chromosome 13 is one of the most frequently altered chromosomes in prostate cancer. The present study was undertaken to examine the role of human chromosome 13 in the progression of prostate cancer. Methods: Human chromosome 13 was introduced into highly metastatic rat prostate cancer cells via microcell-mediated chromosome transfer. Results: Microcell hybrid clones containing human chromosome 13 showed suppression of metastasis to the lung without any suppression of tumorigenicity, except for one clone, which contained the smallest sized human chromosome 13 and did not show any suppression on lung metastasis. Expression of two known tumor suppressor genes, BRCA2 and RB1, which map to chromosome 13, was examined by reverse transcription- polymerase chain reaction analysis. BRCA2 was expressed only in the metastasis-suppressed microcell-hybrid clones, whereas RB1 was expressed in all clones. Conclusion: Human chromosome 13 contains metastasis suppressor gene(s) for prostate cancer derived from rat. Furthermore, the RB1 gene is unlikely to be involved in the suppression of metastasis evident in this system
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